Core Facility
Integrated Bioimaging Facility Osnabrück (IBiOs)
User/Year
100
Usage Guideline

The integrated Bioimaging Facility iBiOs is a core facility within the Center of Cellular Nanoanalytics (CellNanOs), an interdisciplinary Research Center at Osnabrück University. The iBiOs provides access to advanced fluorescence and electron microscopy techniques with highest spatial and temporal resolution. Next to state-of-the-art high-resolution fluorescence imaging techniques, the iBiOs has specialized on live cell single molecule localization microscopy such as PALM/STORM and single molecule tracking as well as single molecule spectroscopy (FLIM, FCS). The iBiOs provides access to cutting-edge techniques such as lattice light-sheet microscopy and develops new nanoparticle based methods such as upconversion microscopy and second harmonic generation microscopy in close collaboration with different research groups at CellNanOs. The EM unit currently applies state-of-the-art 2D scanning and transmission electron microscopy techniques, which are currently extended to 3D and cryo-techniques as well as correlative light-electron microscopy (CLEM). A broad repertoire of labeling techniques is available including the application of nanoparticles in living cells as well as for CLEM for achieving optimum performance.

As a DFG-funded core facility, the iBiOs also provides access to external users.

HEAD OF GROUP

Dr. Rainer Kurre
POSITION Head of Imaging Facility
MAIL rainer.kurre@uos.de
PHONE +49-0541-969-7338

TECHNIQUES

  • Laser Scanning Confocal (LSM)
  • Total Internal Reflection Fluorescence (TIRF)
  • Deconvolution widefield microscopy
  • Single Molecule Imaging Techniques
  • Photoactivated Localization Microscopy (PALM)
  • Functional Imaging of Living Cells
  • Fluorescence Correlation Spectroscopy
  • Probe Microscopy (atomic force or optical)
  • Image Analysis
  • Scanning Electron Microscopy (SEM)
  • Electron Tomography (ET)
  • Correlative Light and Electron Microscopy (CLEM)
  • Immuno-Electron Microscopy
  • Focused Ion Beam Scanning Electron Microscopy (Dual Beam/FIB-SEM)
  • Freeze substitution
  • Microtomy (Cryostat/Vibratome/Paraffin/Resin)
  • Spinning Disc Confocal
  • 2-photon Scanning Confocal (2-P)
  • Single Plane Illumination Microscopy (SPIM)
  • Stochastic Optical Reconstruction Microscopy (STORM)
  • Non-linear Microscopy Techniques
  • Structured Illumination Microscopy (SIM)
  • Transmission Electron Microscopy (TEM)
  • Cryo-Transmission Electron Microscopy (Cryo-TEM)
  • Cryo-Scanning Electron Microscopy (Cryo-SEM)
  • Cryo-Electron Tomography (Cryo-ET)
  • Ultramicrotomy
  • High-Pressure Freezing (HPF)
  • Fluorescence-Lifetime Imaging Microscopy (FLIM)
  • Embedding (Epon/Paraffin/PLT)
  • other
  • Lattice Light-Sheet Microscopy